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主营:RNA干扰(RNAi)研究之相关工具
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当前位置: 首页 > 产品中心 > RNA_interference > SBI/pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression/5 x 10^6 Cells/mL/TCL210C-1-5 x 10^6 Cells/mL
商品详细SBI/pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression/5 x 10^6 Cells/mL/TCL210C-1-5 x 10^6 Cells/mL
SBI/pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression/5 x 10^6 Cells/mL/TCL210C-1-5 x 10^6 Cells/mL
SBI/pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression/5 x 10^6 Cells/mL/TCL210C-1-5 x 10^6 Cells/mL
商品编号: TCL210C-1-5x10^6Cells/mL
品牌: System Biosciences
市场价: ¥30620.00
美元价: 18372.00
产地: 美国(厂家直采)
公司:
产品分类: RNA干扰
公司分类: RNA_interference
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Human Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression, you can choose between three different ways to study Foxp3 overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:

Lentivector-based for transfection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your Foxp3 overexpression cassette to cells using transduction—great for transfection-resistant cell lines.

PiggyBac Transposon-based* for infection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for Foxp3 overexpression in infection-resistant cell lines.Pre-built Jurkat cell line to save time and labor

Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the MSCV-Human Foxp3-EF1α-GFP-T2A-Puro expression cassette and have validated Foxp3 overexpression, so you don’t have to—just culture cells and start your studies.

*Customer AgreementsAcademic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.For end user license information, see the following:

  • Academic Customers—End-User License Information
  • Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

How It Works

A quick introduction to SBI’s lentivector packaging and PiggyBac Transposon technologies

Start producing lentivirus

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

 

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.1. The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.2. The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.3. The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Supporting Data

Robust overexpression of Foxp3

Figure 2. SBI’s human and mouse Foxp3 lentivector and PiggyBac plasmids provide robust Foxp3 overexpression. The human and mouse Foxp3 lentivectors Cat.# TCL200A-1 (human) and Cat.# TCL100A-1 (mouse) and the PiggyBac transposon constructs Cat.# TCL200PB-1 (human) and Cat.# TCL100PB-1 (mouse) were transiently transfected into 293FT cells. After 24 hours, cellular lysates were prepared and Western blot analyses performed to detect the Foxp3 protein expression using an anti-Foxp3 antibody that detects both human and mouse proteins. Robust overexpression of both human (lanes 1,3) and mouse (lanes 2,4) Foxp3 proteins compared to the non-transfected cell lysate control (lane 5) can be clearly observed, with tubulin as a loading control.

Figure 3. SBI’s MSCV-Human Foxp3-EF1α-GFP-T2A-Puro Jurkat Cell Line shows strong Foxp3 overexpression. Human Jurkat T cells were transduced with either Cat.# TCL200A-1 (human) or Cat.# TCL100A-1 (mouse) pre-packaged lentivirus and stable cell lines established using puromycin selection for seven days. Overexpression of Foxp3 was evaluated by measuring GFP levels using flow cytometry and Western blot analysis of cellular lysates and using anti-Foxp3 antibody. Both methods show that SBI’s Foxp3-overexpressing Jurkat Cell Line possesses strong Foxp3 overexpression.

Resources

User Manual: Treg and Th17 T cellImmunology Tools
Protocol: Spinoculation of Suspension Cells
Lentivirus Safety Guidelines
Product Sheet: Immunology Research – T Cell Tools
Brochure: Gene Delivery and Expression Products and Services
品牌介绍
美国SBI代理 System Biosciences,简称SBI,美国加州湾区新成立的生技公司,致力于独特、创新生物技术之开发,以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰(RNAi)研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术,为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司,提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1
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